Rajeshkumar et al. World Journal of Pharmacy and Pharmaceutical Sciences. ANTICANCER ACTIVITY OF ANDROGRAPHIS PANICULATA. showing anticancer activity on HEp2 – Human larynx carcinoma cell lines in-vitro KEYWORDS: Andrographis paniculata,Herbal medicine. A refined extract of A. paniculata, also significantly reduces activities of lipid .. The anticancer activity was observed due to the presence of andrographolide.
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How to cite this article: There were strong relationships between most studied traits. These natural products are supposed to minimize DNA damage by reacting with free radical s and in this way they can prevent cancer.
Interestingly, painculata correlation between proline as well as NAG with the other measured morphological characteristics was significant and paniculats in the salt-stressed plants. So, here we are using the natural drugs from phytochemicals to reduce the toxicity level. The antioxidant phytochemicals protect the cells from oxidative damage. The viable cells were counted using a Coulter actuvity and diluted with RPMI medium and final densities of x10 4 cells mL -1 were obtained.
The results showed that proline, AG, and NAG contents were positively correlated to the substrate concentration of NaClwhile the total crude extract amount was negatively correlated to salinity levels Figure 2.
This can be utilized as a potential in the next breeding programs to develop the salt-tolerant varieties in A. One mL of each sample was finally filtered into HPLC vials using disposable polypropylene syringe filters with 0. The plants grown under salinity conditions performed different responses after four weeks of salt treatment. Mostly drugs are used anticabcer restore the function of tumor supressor genes. The results obtained suggest that Andrographiw. Whatman filter paper no.
However, the use of salinity to enhance the biosynthesis of the phytochemicals must be pamiculata regarded, and it should not be forgotten that the increased concentration of bioactive compounds such as AG, NAG, and DDAG by salt stress in general is associated with a reduction of biomass production.
The day seedlings were transferred from jiffy media into the pot with sand medium. Phenols, polyphenols, flavonoids and their derivatives are ubiquitous in plants and have been found associated with the inhibition of atherosclerosis and cancer Cirla and Mann, Similarly, alkaloids like schischkinnin and montamine have been isolated from the seeds of Centaurea schischkinii and Centaurea montana which showed anticancer property Yang and Wang, This event complies with the hypothesis that the A.
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The results indicated that salinity had a significant effect on the aforementioned three anticancer phytochemicals. Hepatoprotective and immunological effects of antioxidant drugs. Increases in certain amino acids including prolinesugars including sucrose, fructose, and glucoseand polyols including inositols have been reported in some plant species like grapevine [ 7 ], Limonium latifolium [ 8 ], and Lotus japonicas [ 9 ]. panicuata
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Under the low level of salinity, the salt ions inhibited the biosynthesis of phytochemicals as indicated by the decrease in their amounts in both tolerant and sensitive accessions. ME at the ratio of 1: The herb exhibited a wide scope of pharmaceutical properties such as anti-HIV [ 19 ], anti-H1N1 [ 20 ], anticancer [ 21 ], antihepatitis [ 22 ], anti-inflammatory [ 23 ], blood purifier, and antidiarrhea [ 24 ].
Analysis of variance showed high significant differences among the accessions for AG and NAG contents. Natural drugs have less toxicity andrographix the commercially available drugs. On the basis of measurement of OD, the anticancer study suggest that S. After every three salinity applications, plants were again irrigated with normal Hoagland nutrient solution. PDF Kb Abstract Plant-derived compounds act as a prevention within the development of many anti-cancer agents.
However, the standard curves were compared for each sample and the reliability of the peaks was confirmed accordingly. Journal of Pharmacology and Toxicology, 8: Effects of salinity levels on TDW, proline and three main phytochemicals in tolerant and sensitive accession of A.
List of the 4 accessions of Andrographis paniculata collected from Antixancer Malaysia. Many of those metabolic changes such as sucrose, oligosaccharides, polyhydric alcohols, proline, and polyamines are observable in the leaves of plants exposed to abiotic stresses [ 4 ].
However, this should not be interpreted in the way that saline water and soil are suggested as an alternative solution to increase the production of A.
In other words, the increase of these phytochemicals under salinity condition would be partially compensated by a decline in total biomass. Since the highest amount of the active components is found just before the plant blooms [ 39 ], the day-old plants were subjected to different salinity levels.
After four weeks, a significant decrease in growth driven by salinity was apparent in all accessions. Again, the combination 1: Culturing of cell line: All chemicals used were of analytical grade. In other words, under salinity stress, the tolerant accessions were capable of accumulating the higher amounts of proline, AG, and NAG than the sensitive accessions.
Accession showed a growth reduction of dry weight up to The correlative aspects of the three anticancer phytochemicals production as well as the accumulation of proline in the tolerant and sensitive A. In this regard, our results indicated that proline as well as the AG and NAG contents is positively correlated with the substrate concentration of salt, in which the tolerant accessions were higher in proline, AG, and NAG contents compared with the sensitive ones.
Ten Hoopen, and J. Further studies are required to isolate the active constituents involved in the antioxidant and hepatoprotective activity of the plant. The process was repeated several times with the same solvent system until the solvent portion becomes colorless.
Analysis of the Anticancer Phytochemicals in Andrographis paniculata Nees. under Salinity Stress
Fundamentally, it can be concluded that if the production of anticancer phytochemicals of A. The effects of salinity levels on studied traits among the four accessions of A. Effect of silymarin on different acute inflammation models and on leukocyte migration. There are several commercially available HDAC inhibitors but they are highly toxic in nature. The materials were soaked for three days at room temperature. View at Anticamcer Scholar.